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KMID : 1036720150480060468
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Journal of Nutrition and Health
2015 Volume.48 No. 6 p.468 ~ p.475
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Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children
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신윤경:Shin You-Kyung
최지원:Choi Ji-Won/오세영:Oh Se-Young/정자용:Chung Ja-Yong
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Abstract
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Purpose: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers.
Methods: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8-hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA.
Results: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA.
Conclusion: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.
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KEYWORD
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Genetic polymorphism, Mn-superoxide dismutase, glutathione S-transferase, 8-hydroyxyl-2-deoxyguanosine
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